Journal: Experimental & Translational Stroke Medicine

Article Title: Neuronal precursor cell proliferation in the hippocampus after transient cerebral ischemia: a comparative study of two rat strains using stereological tools

doi: 10.1186/2040-7378-2-8

Figure Lengend Snippet: Infarct distribution in SDs and SHRs . Representative examples are shown of the infarct distribution after 90 minutes of tMCAo in the SD and SHR strains using NeuN and ED-1 immunohistochemistry. The ED-1 (brownish red rows) was visualized with NovaRed ® , whereas NeuN stain was developed with nickel-enhanced DAB (grey rows). A and B depict the infarct pattern we found in SDs. Usually, the infarcts were restricted to the subcortical structures in SDs encompassing both the MCA and AChA/HTA territories (4 SDs) ( A ) or the AChA/HTA territories alone (5 SDs) ( B ) Three of the tMCAo SDs had no histological signs of neuronal loss on the NeuN stain. C , D and E illustrate the infarct distribution in SHRs. In most of the SHRs subjected to ischemia the infarcted area included the neocortex (8 out of 11 SHRs). C shows an example of ischemia affecting the ACA, MCA and AChA/HTA (2 SHRs). The majority of animals had ischemia in the MCA and AChA/HTA territories (8 SHRs) ( D ). Only one SHR had a small ischemic lesion solely affecting the AChA/HTA territories ( E ). ACA , anterior cerebral artery; AChA , anterior choroidal artery; DAB , 3,3'-diaminobenzidine; HTA , hypothalamic artery; MCA , middle cerebral artery; NeuN , neuronal nuclei; SDs , Sprague-Dawley rats; SHRs , spontaneously hypertensive rats; tMCAo , transient middle cerebral artery occlusion.

Article Snippet: The sections were finally incubated with an avidin-biotin-peroxidase complex kit (Cat. no. PK-6100, Vector Laboratories, Burlingame, CA, USA) for 1 hour at room temperature before the development with nickel-enhanced DAB or NovaRed ® (Cat. no. SK-4100 and SK-4800, Vector Laboratories, Burlingame, CA, USA).

Techniques: Immunohistochemistry, Staining